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Txhua pawg

project

project

D-pantolactone yog qhov tseem ceeb chiral intermediate rau synthesis ntawm D-pantothenic acid series. Nws yog tsuas yog siv rau kev sib txuas ntawm pantothenic acid (vitamin B5) lossis calcium ntsev, D-panthenol (vitamin B5), D-pantoethylamine thiab lwm yam khoom lag luam.

Peb lub tuam txhab txais kev tsim kho tus kheej tsim microbial caj ceg engineering kab mob L-lactonohydrolase ua cov kab mob thiab cov txheej txheem ua ntej thev naus laus zis thiab immobilized cov cab kuj npaj tshuab, thiab npaj D-pantolactone los ntawm txoj hauv qab no los npaj D-calcium pantotheate:


Los ntawm ze li ntawm ib xyoos ntawm kev tsim kho thev naus laus zis thiab cov txheej txheem txhim kho, kev kub siab fermentation tam sim no siv nyob rau hauv E. coli qhia system, thiab cov haujlwm fermentation tuaj yeem ncav cuag 15000 U / L, thiab ruaj khov thiab txhim khu kev qha enzyme rho tawm, kev ua kom huv, kev hloov kho thiab immobilization kab tau txiav txim siab. Lub cev ua kua enzyme muaj purity siab, siab immobilization kev ua si, lub zog zoo, kev ua haujlwm zoo ntawm kev ua haujlwm, kev ua haujlwm siab hauv cov txheej txheem enzymatic npaj ntawm D-pantolactone, cov txheej txheem yooj yim thiab cov khoom lag luam zoo. Los ntawm kev ua kom zoo ntawm kev npaj thev naus laus zis rau kev qhia cov tsheb thauj mus los hauv immobilized, cov thev naus laus zis npaj technology kuj tau ua qhov tseem ceeb hauv cov txheej txheem thiab nqi. Los ntawm kev ntsuas me me thiab nruab nrab, qhov kev ua haujlwm hauv immobilization tuaj yeem ncav cuag ntau dua 100U / g, thiab lub voj voog hloov hloov mus los yuav suav txog txog 100 lub cev raws li kev ntsuas qog ua qoj ib puag ncig.