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D-pantolactone ke karolo ea bohlokoa ea bohareng ea chiral bakeng sa motsoako oa li-mfululizo oa D-pantothenic acid. E sebelisoa haholo-holo bakeng sa motsoako oa pantothenic acid (vithamine B5) kapa letsoai la calcium, D-panthenol (vithamine B5), D-pantoethylamine le lihlahisoa tse ling.

Khampani ea rona e amohela baktheria ea boenjiniere ea liphatsa tsa lefutso tse iketselitseng L-lactonohydrolase e hlahisang libaktheria le theknoloji ea ts'ebetso ea pele le theknoloji e sa sebetseng ea bakuli, 'me e lokisa D-pantolactone ka tsela e latelang ho lokisa D-calcium pantotheate:


Hoo e ka bang selemo se le seng sa nts'etsopele ea mahlale le ts'ebetso ea ts'ebetso, ts'ebetso e phahameng ea methapo e ntse e sebelisoa lenaneong la polelo ea E. coli, mme ts'ebetso ea matlafatso e ka fihlela 15000 U / L, le tšibollo e tsitsitseng le e ka tšeptjoang ea enzyme mohala o ikemiselitse. Mokelikeli oa enzyme o na le bohloeki bo phahameng, ts'ebetso e phahameng ea ho emisa, matla a mangata, ts'ebetso e ntle ea filtara, katleho e phahameng ts'ebetsong ea ho hlophisoa ha enzymatic ea D-pantolactone, ts'ebetso e bonolo le boleng bo botle ba sehlahisoa. Ka ts'ebetso ea thekenoloji ea tlhophiso ea ho hlahisa batho ba sa tsamaiseng thepa, theknoloji ea ho lokisa thepa e boetse e hlahisitse katleho e kholo tšebetsong le ka theko. Ka tlhahlobo e nyane le e mahareng, ts'ebetso ea ho thibela bokhachane e ka fihla ho feta 100U / g, mme potoloho ea potoloho ea potoloho e ka baloa ho fihlela li-batches tse 100 ho latela sekhahla sa ts'ebetso ea enzymme.